Medical Laboratory Journal

en Evaluation of the effect of sclareol on endoplasmic reticulum stress in the KATO III human gastric carcinoma cell line ژنتیک انسانی Human Genetics Research Article Research Article <div style="text-align: justify;">Background: The main goals of chemotherapy are to induce apoptosis (Cell death) and arrest the cell cycle in cancer cells. An important factor in inducing apoptosis in cancer cells is increased endoplasmic reticulum stress. This study investigated the effect of Sclareol on the ATF6 pathway, as well as its effects on two other genes, VEGF and E2F1, associated with the UPR pathways. Methods: The target population comprised KATO III human gastric carcinoma cells purchased from the Pasteur Institute of Iran. The cells were cultured in the cell culture laboratory until their number reached at least 100,000 cells/well. They were treated with 0, 20, 40, 60, 80 and 100 μM Sclareol for 5 hours. The expression of the genes specified in the research objectives was analyzed in gastric cancer cells before and after Sclareol treatment using quantitative real-time PCR on cell-line spheroids cultured in suspension. SPSS Software was used for statistical analysis. Results: ATF-6 gene expression increased significantly at Sclareol doses of 20, 40 and 60 μM (P<0.0001). In contrast, VEGF gene expression decreased significantly at Sclareol doses of 20, 40 and 60 μM (P<0.001). E2F1 gene expression also increased significantly at Sclareol doses of 20, 40 and 60 μM (P<0.003). Conclusion: The findings of the present study suggest that Sclareol may reduce VEGF gene expression and increase the expression of ATF-6 and E2F1, which are responsible for increasing endoplasmic reticulum stress in KATO III human gastric cancer cells. Sclareol doses of 40 and 60 μM were considered effective.</div> Gastric Cancer, Endoplasmic Reticulum Stress, Sclareol, KATO III Gastric Cancer Cells 0 0 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1835-1&slc_lang=en&sid=1 Mojtaba Ghaffarian Gabalou benzbmw142@gmail.com 0009-0000-7329-0991 No Department of Molecular Genetics, Ah.C., Islamic Azad University, Ahar, Iran Seyed Ali Rahmani rahmaniseyedali@yahoo.com 0000-0002-4174-6437 Yes Department of Molecular Genetics, Ah.C., Islamic Azad University, Ahar, Iran Changiz Ahmadizadeh Dr_ahmadizadeh@yahoo.com 0000-0003-0076-2446 No Department of Microbiology, Ah.C., Islamic Azad University, Ahar, Iran Rasoul Sharifi rasoulsharifi.sci@gmail.com 0000-0001-5079-5655 No Department of Biochemistry, Ah.C., Islamic Azad University, Ahar, Iran