Search published articles


Showing 3 results for Placenta
Effect of Mouse Liver Extract on in Vitro Differentiation of Amniotic Membrane Stem Cells into Hepatocyte-Like Cells
Vahide Vahideh Assadollahi , Masoume Jalalvand, Shahrokh Bagheri, Hamed Esmaiel Lashkarian ,
Volume 10, Issue 6 (11-2016)
Abstract

ABSTRACT

          Background and Objective: Multipotent placental amniotic membrane mesenchymal stem cells (MSCs) are capable of differentiating into specialized tissues under different conditions. The aim of this study was to induce differentiation of placental amniotic membrane MSCs from NMRI mouse into hepatocytes using liver extract.

         Methods: Placental amniotic membrane MSCs from a 14-day pregnant female mouse was used in this study. The cells were incubated with trypsin solution, followed by pipetting. The resulting suspension was cultured in 12-well plates. After confirming their mesenchymal nature, differentiation of the aforementioned cells was induced via exposure to 6, 18, 30 and 60 μg/ml of liver extract. On the 16th day of treatment, immunocytochemical reaction for albumin and periodic acid-Schiff (PAS) test were performed for detection of hepatocyte-like cells.

          Results: Change was observed in the shape of differentiating cells from spindle-like shape to polygonal shape. The immunocytochemical reaction of the differentiated cells was positive. PAS staining also confirmed the accumulation of glycogen particles in the aforementioned cells. Concentration of 6 μg/ml liver extract was found as the effective dose for induction of differentiation.

           Conclusion: The findings of this study show that the placental amniotic membrane-derived MSCs of mouse can differentiate in vitro from spindle-like cells to polygonal hepatocyte-like cells with large nuclei and under the influence of the liver.

Keywords: Placental Amniotic Membrane Mesenchymal Stem Cells, Hepatocyte, In Vitro.


The effect of swimming and silymarin on placental growth factor in pregnant rats exposed to cadmium
Shadmehr Mirdar, Monireh Khalili , Neda Aghaei Bahmanbeglou ,
Volume 17, Issue 5 (9-2023)
Abstract
Background: Exercise and dietary supplements can partially mitigate the negative effects of cadmium. The present study aimed to investigate the effect of swimming and silymarin on placental growth factor (PLGF) in pregnant mice exposed to cadmium.
Methods: Seventy-two 8-week-old pregnant Wistar rats (weighing 20 ± 200 g) were divided into 9 groups, with 8 rats in each group. Cadmium chloride at a dose of 400 mg/kg body weight was fed to rats by drinking a water solution. Silymarin (100 mg/kg body weight) was injected subcutaneously 3 times a week. The exercise program during pregnancy consisted of 60 minutes of swimming per day, conducted for 5 days a week. The microscopic sections of samples were taken 2 days after birth using the usual method of tissue sectioning. A 1-way analysis of variance (ANOVA) and Tukey post hoc test at the error level of 0.05 were used to analyze the data.
Results: The PLGF index in the cadmium group showed a significant decrease (P < 0.001) compared to the cadmium + silymarin and cadmium + silymarin and swimming groups. However, swimming training alone had no effect on PLGF index (P = 0.162).
Conclusion: Cadmium significantly reduced PLGF levels in neonatal lung tissue, and regular swimming endurance exercises and silymarin supplementation inhibited the effects of cadmium chloride.
The pathophysiological spectrum of maternal complications of pregnancy-induced hypertension: Review Article
Adedeji Okikiade , Chidinma Kanu , Oluwadamilare Iyapo , Ololade Omitogun,
Volume 19, Issue 3 (7-2025)
Abstract
Background: Pregnancy-induced hypertension (PIH) is a multi-system disorder affecting 6-8% of pregnancies in the U.S. and contributing significantly to maternal mortality, accounting for 16% in developed countries. It progresses from preeclampsia to eclampsia, leading to multi-organ damage through mechanisms such as oxidative stress, placental ischemia, and endothelial dysfunction. While the exact pathogenesis remains unclear, genetic, immunologic, and environmental factors are implicated. The American College of Obstetricians and Gynecology (ACOG) recommends initiating treatment when diastolic blood pressure exceeds 105-110 mmHg.
Methods: This narrative review examines existing literature on PIH, including epidemiological data, pathophysiological mechanisms, clinical management guidelines, and associated complications such as abnormal placentation, oxidative stress, and endothelial dysfunction.
Results: This study demonstrates that hypertensive disorders of pregnancy (HDP) significantly impact maternal and fetal health, particularly in developing countries with limited healthcare access. Early detection and continuous monitoring play a key role in reducing complications. Additionally, HDP is associated with increased long-term cardiovascular and metabolic risks, highlighting the importance of postpartum follow-up.
Conclusion: HDP poses a serious threat to maternal and fetal health, with potential long-term consequences. Effective management requires early diagnosis, close monitoring, and postpartum follow-up. Global implementation of risk assessment and targeted care strategies can help reduce the burden of this condition. Strengthening healthcare systems and increasing awareness among healthcare providers and patients are essential steps toward improving outcomes.

Page 1 from 1     

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.