Showing 5 results for Nosocomial
A R Abdolahi,
Volume 3, Issue 2 (10-2009)
Abstract
Abstract Background and objectives: A Nosocomial infection is one of the Leading problems causing high mortality among hospitalized patients. This study aimed at confirming the concurrence of Nosocomial infections with microorganisms spreading in the air of hospital wards. Material and Methods: This study was conducted in 2009 at ValieAsr Hospital in Tehran, Iran. The Samples were taken from the air of different sections of the hospital both actively (with Quick-Tak, 30) and passively. After that, the samples were investigated for bacerial and fungal contamination and the results were compared with the results of Laboratory Studies of patients in different wards. Results: The Bone Marrow Transplant (BMT) ward shows the least fungal and microbial contamination whiles the Intensive Care Unit (ICU) the highest. The fungi are essentially Cladosporium and penicillium while the most well-Known organisms are Micrococcus and Staphylococcus epidermidis. Stenotrophomonas is seen in both blood and air culture in thorax surgery ward. Also, the concurrence of Staphylococcus epidermidis in the samples of the air and patients is considerable. Conclusion: There isn’t concurrence between the Nosocomial caused organism, apart from Stenotrophomonas and Staphylococcus epidermidis, and microorganisms isolated from patients. Because the bacterial and fungal organisms are in the air of Hospital, it is a necessity to install proper ventilation system. Keywords: Air Bacterial Contamination, Hospital, Nosocomial Infection
F Sadighian, Z Abedian, A Saane-Ei, Z Mirkarimi,
Volume 7, Issue 3 (10-2013)
Abstract
Abstract
Background and Objective: Nowadays, nosocomial infection is one of the greatest problems in hospitals. Normal flora of staff’s hands and the bacterial agents on the surface of medical equipment can become progressively colonized with potential pathogens during patient care. This study was carried out to determine the bacterial agents existed on staff’s hands and in the wards of hospital to step in to control nosocomial infection.
Material and Methods: In this descriptive study, during 17 months (22.mar.2010- 30.aug.2011), 403 samples, using sterile swab , were randomly obtained from the staff’s hands and medical equipment of emergency departments , ICU, male operation room and female surgical unit . The samples were cultured on Blood agar (BA) and Eosin methylene blue (EMB). Then, identification of isolated bacteria was done with diagnostic tests.
Results: Of 430 samples, 530 bacteria were isolated from staff’s hands (N= 291) and medical equipment (N= 234). The most common bacterium from personnel’s hands (144 49.5%) and medical equipment (24 10%) is Staphylococcus aureus. Also, three isolates of pseudomonas aeruginosa from staff’s hands of male surgical ward and medical equipment of ICU, and two isolates of Acinetobacter.spp from ICU’s medical equipment were identified.
Conclusion: With regard to the findings, it seems that applying the appropriate disinfectant agents by using standard procedures is necessary.
Keywords: Medical Equipment Staff’s Hand Nosocomial Infection Staphylococcus Aureus
A Hajihasani, M Douraghi, M Rahbar, M Mohammadzadeh, H Zeraati, S Ghoorchian, M Alavi Moghadam, M Sabzi,
Volume 8, Issue 2 (7-2014)
Abstract
Abstract
Background and Objective: Stenotrphomonas maltophilia is an opportunistic nosocomial pathogen with high mortality in immunocompromised cases. The aim of this study was to isolate and identify Stenotrphomonas maltophilia in the hospitals’ environment and wards.
Material and Methods: In this cross-sectional study, a total of 1108 samples were collected from environment of two hospitals during 12 months. Identification of isolates was performed using biochemical, phenotypic (intrinsic resistance to carbapenems) and molecular methods (amplification of 23S rRNA gene).
Results: Of the studied samples, 186 (16.78%) nonfermentative gram negative bacilli (NFGNB) were identified. Amongst NFGNB, 18 (1.62%) isolates were identified as S. maltophiliaby using biochemical tests. Of 18 biochemically identified isolates, 15 (83.3%) were confirmed via PCR. Sinks (40%) and men surgery ward ( 33.3 %( were the most contaminated sites and wards of hospitals, respectively.
Conclusion: S. maltophilia is repeatedly isolated from sink which shows that the moist hospital environments need to be considered as a source for dissemination of bacteria.
Keywords: Nosocomial Infections, Nonfermentative Gram Negative, Stenotrphomonas Maltophilia, PCR
M Elmi Merian, M Faezi Ghasemi,
Volume 8, Issue 3 (8-2014)
Abstract
Abstract
Background and Objective: Burkholderia cepacia complex (BCC) is a plant pathogen that is an important mortality factor in immune-compromised and hospitalized patients. We aimed to Isolate and Characterize the Burkholderia Cepacia Strains from Hospitalized Patients in the Hospitals of West Guilan Province.
Material and Methods: This study was conducted on 90 saliva and blood samples obtained from patients with blood infection, pneumonia, asthma, patients connected to the monitoring and ventilator systems, and immune-compromised patients in different sections of hospitals such as the pediatrics, internal section, ICU and CCU. Primary screening was performed by cultivating the samples on Burkholderia cepacia selective agar (BCSA) Sensitivity to antibiotics was tested by Kirby-Bauer and Muller-Hinton Agar (MHA) and the separated isolations were recognized by strengthening the gene rec A.
Results: Of 90 isolated samples, only one strain suspected B. cepacia was isolated from 24-year old women with asthma. This strain was resistant to bacitracin, pipracillin and ciprofloxacin antibiotics.
Conclusion: The incidence of B. cepacia is rare in western part of Guilan province, which is congruent with the results of overall incidence in Iran.
Shayosree Sarkar, Sonal Chavan, Geetika Agrawal, Heena Rahangdale, Sunanada Zodpey,
Volume 19, Issue 2 (3-2025)
Abstract
Background: Burkholderia cepacia complex is are opportunistic nosocomial pathogen that can cause severe infections in neonates, involving the respiratory tract, the urinary tract and bloodstream infections. Therefore, it can lead to outbreaks through different sources. This study was conducted with the aim of early detection and successful control of an outbreak caused by Burkholderia cepacia complex.
Methods: A cross-sectional study was conducted in a tertiary care hospital over a one-month period, July 2023. Blood culture samples of 11 neonate’s yielded growth of non-fermenting, oxidase-positive and motile, Gram-negative bacilli. Isolates were provisionally identified to be Burkholderia cepacia complex by conventional biochemical tests and antimicrobial susceptibility patterns. The increased, repeated, and continuous isolation of the same isolate raised the suspicion of an outbreak in the neonatal intensive care unit. Active surveillance was undertaken to trace the source and contain the bacteria. Identification of isolates was confirmed by VITEK 2 (BioMérieux, France) compact microbiology analyser.
Results: Surveillance revealed sources of Burkholderia cepacia complex for all 11 neonates. Sources of infection could be traced to intravenous catheters and cradles of the neonates and operation theatre beds, and instrument trolleys of the labour room where the babies were delivered. All the environmental isolates showed strain-relatedness of Burkholderia cepacia complex with the clinical isolates, along with a similar antibiotic susceptibility pattern. Timely interventions aided in the control of the outbreak.
Conclusion: This study presents the importance of the hospital infection control team in the management of an outbreak of Burkholderia cepacia complex in neonates.
