Showing 42 results for Pcr
Masoomeh Rezanezhadi, Fahimeh Azadi, Shayan Marhamati, Hamid Reza Joshaghani,
Volume 17, Issue 6 (11-2023)
Abstract
Background: Herpes simplex viruses (HSV-1 and HSV-2) are a common cause of dermal, oral, and genital infections worldwide. Rapid diagnosis of infected patients will prevent transmission to uninfected individuals. Therefore, this study aimed to detect HSV-1 and HSV-2 by real-time polymerase chain reaction (RT-PCR) in patients from Golestan, Iran.
Methods: In this cross-sectional study, 645 suspected HSV patients hospitalized in the health centers of Golestan Province were selected between 2015 and 2020. Demographic data were collected, and DNA was extracted from the samples. Detection of HSV-1 and HSV-2 was performed by RT-PCR.
Results: Of the 645 samples tested by RT-PCR, 9 samples (1.39 %) were found to be positive for HSV-1/HSV-2, and the rest of the samples (98.6%) were negative. Of the 9 positive samples, 8 cases (88.8%) were HSV-1, of which 7 cases (77.7%) were from the cerebrospinal fluid (CSF) and 1 case (12.5%) from the vagina. HSV-2 was seen only in 1 vaginal specimen (11.1%).
Conclusion: Early diagnosis of these viral infections is very important as it can lead to early treatment and avoid further symptoms and even death. Thus, molecular approaches can provide an option for quicker diagnosis.
Fatemeh Livani , Faramarz Koohsar , Farideh Tohidi, Mitra Sharbatkhori , Roghiyeh Faridnia , Ganesh Yadagiri , Ayeneh Hajieh Pangh , Mehdi Khoshrou , Hamed Kalani ,
Volume 19, Issue 1 (1-2025)
Abstract
Background: Cutaneous leishmaniasis (CL) is a prevalent infectious zoonotic disease between humans and animals. Golestan Province is one of the important centers of CL in Iran. The current method for detecting Leishmania parasites in patients is Giemsa-stained direct smear from skin ulcers; however, PCR is a strongly recommended method for epidemiological studies. This study aimed to investigate Leishmania species in negative ulcer smears from patients suspected of having CL referred to Aq Qala Health Center using the multiplex nested PCR method.
Methods: This study was performed on 72 negative ulcer smears from patients suspected of having CL referred to Aq Qala Health Center, Golestan Province, northeastern Iran, from August 2019 to April 2020, using the multiplex nested PCR method to detect Leishmania major and Leishmania tropica species.
Results: Out of 72 samples, 4 (5.55%) samples were positive by multiplex nested PCR. Moreover, all positive samples are related to Leishmania major species.
Conclusion: The multiplex nested PCR method is strongly recommended for detecting Leishmania species in negative ulcer smears from patients suspected of having CL.
