Ghadir Sohrabi, Mahdi Malmir, Ali Asghar Ghafarizade, Tayebeh Faraji,
Volume 9, Issue 1 (3-2021)
Abstract
Background and Objective: Downscale outcome in assisted reproductive techniques (ART) is associated with lipid peroxidation. Melatonin may have beneficial effects against lipid peroxidation and oxidative stress. This work was aimed to evaluate the ameliorative capacity of melatonin versus the passage of time and lipid peroxidation on sperm parameters from the men with ATS in the ART process.
Material and Methods: Semen samples were collected from 50 asthenoteratozoospermic (ATS) men. Samples were divided into control and melatonin group. The test group were incubated with 6 mM melatonin at 2, 4, 6, and 24 hours. Then total and progressive motility, membrane integrity, and Malondialdehyde levels (MDA) were evaluated. Statistical analysis was carried out by SPSS software (repeated-measures ANOVA).
Results: In the control group, total (p<0.01) and progressive (P<0.05) motility and also, membrane integrity (P<0.03) significantly decreased although, MDA levels of sperm significantly increased (*P<0.04). Moreover, in the melatonin group, the mentioned parameters significantly compensated compared to the control group (P<0.05).
Conclusion: In the outcome, melatonin in vitro treatment paves the way for motility and membrane integrity up-regulating in the ATS men.
Samira Naderi Noreini, Roya Ahmadi Nia Tabesh, Mahdi Malmir, Ali Asghar Ghafarizade,
Volume 11, Issue 2 (10-2023)
Abstract
Background: Sperm preparation is a time-consuming process that makes a contribution to oxidative stress induction in assisted reproductive techniques.
Objective: This study aimed to evaluate the effect of L-carnitine on sperm parameters during semen preparation at different time intervals.
Materials and Methods: In this in vitro study, the semen samples of 50 asthenoteratozoospermic men were divided into the control and the L-carnitine groups (incubated with 0.5 mg/mL L-carnitine) (n=25/each). Total motility, progressive motility, sperm membrane integrity (by Hypo-osmotic swelling test), and lipid peroxidation (by Malondialdehyde level) were assessed in each group after 2, 4, and 6 hours. Afterward, samples were analyzed by the World Health Organization (2010) criteria.
Result: The results showed that in the control group total and progressive motility, and membrane integrity of the sperm were significantly decreased after 2 hr (P= 0.028, p= 0.019, and p= 0.025, respectively), while Malondialdehyde levels increased significantly after 4 hr (P= 0.018). Sperm parameters in the L-carnitine group increased significantly after 2 hr (P= 0.002), while Malondialdehyde levels decreased significantly compared to the control group in 6 hr (P < 0.001 vs. P =0.045).
Conclusion: Based on these results, L-carnitine can manage to conserve motility and sperm membrane integrity of sperm from the hazardous impact of oxidative stress and lipid peroxidation during sperm preparation.
