Showing 8 results for Extract
Dr Behrooz Yahyaei, Dr Mahnaz Nouri, Dr Hamid Matmir,
Volume 6, Issue 1 (3-2018)
Abstract
Background and objectives: Anabolic steroids are routinely consumed by athletes. Therefore, this study was conducted to investigate the effect of Boldenone (BOL) on testicular tissue and the healing effect of jujube (Ziziphus jujuba) hydroalcoholic extract in addition to physical exercise.
Methods: In the present case–control study, 42 male wistar rats were purchased and divided into 3 experimental groups. 28 rats were subjected to BOL injection and subsequently divided into 4 groups. The control group, Sham and 8-week BOL group which was also divided into 4 subsequent groups of controlling BOL complications after 8 weeks, untreated control, jujube extract and jujube extract along with physical exercise.
Results: In the present study we found that jujube extract exerted healing effects on all groups pf treated rats in addition to the exercise training groups.
Conclusion: Jujube extract along with physical exercise may exert healing effects on testicular tissue after administration of anabolic BOL steroid.
Zahra Roustaeizade, Mohamad Hassan Akhavan Karbassi, Khatereh Kheirollahi, Ehsan Babaei Zarch,
Volume 6, Issue 3 (9-2018)
Abstract
Background and objectives: Recurrent aphthous stomatitis (RAS) is a painful ulcerative lesion and its incidence is 20% in the society. Myrtus communis (myrtex) has been effective in the treatment of RAS. In this study, two different concentrations (2.5% versus 5%) of myrtex extract were evaluated to discover the most effective concentration for the treatment of RAS.
Methods: In this randomized, double-blind clinical trial, 60 patients with RAS were evaluated. Thirty patients used myrtex extract 5% and thirty patients used myrtex extract 2.5% (10 drops on lesion for 20 seconds 5 times per day). The severity of pain and burning sensation experienced by patients were measured by visual analogue scale (VAS) and the size of the lesion was estimated by transparent calibrated grid Data analysis was done by running t-test and repeated measures statistical test.
Results: The mean of the largest RAS diameter before treatment was decreased in both groups 1 day and 7 days after treatment (P-value=0.000); however, these differences were not significant in both groups (P-value =0.401). Furthermore, the severities of pain and burning sensation were decreased in both groups (p-value = 0.000). Nevertheless, this decrease was similar in both groups (P-value = 964).
Conclusion: Treatment with different concentrations of myrtex extract is effective in decreasing RAS diameter, pain, and burning sensation. In addition, the therapeutic efficacy of two different concentrations of myrtex extract (2.5% and 5%) was similar in this regard.
Abolfazl Jafari-Sales, Parisa Bolouri,
Volume 6, Issue 4 (12-2018)
Abstract
Background and objectives: Today, due to the increasing antibiotic resistance of bacteria, the use of medicinal plants as a suitable alternative to antibiotics has increased significantly; therefore, in this study, the antibacterial effects of methanolic extracts of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli were evaluated.
Methods: In this laboratory research, after collecting plants and confirming its scientific name, extract of Glycyrrhiza glabra L. Root was prepared by Soxhlet extractor method at concentrations of 20 mg / ml to 400 mg / ml. Then the antimicrobial effects of this extract were investigated using Agar well diffusion and Dilution test methods.
Results: The results showed that the methanolic extracts of G.glabra L. in both Agar well diffusion and Dilution test methods had antibacterial effects on the tested bacteria. The highest effect was observed on S.aureus and the lowest effect was observed in P.aeruginosa.
Conclusion: According to the above results, it can be expected that the G.glabra L. extract can be used to treat bacterial infections and is a suitable alternative to commonly used chemical treatments for the treatment of infections.
Javad Sayyahi, Hayedeh Mobaiyen, Behboud Jafari, Abolfazl Jafari-Sales,
Volume 7, Issue 3 (9-2019)
Abstract
Background and objectives: As much as people become aware of the dangerous side effects of synthetic antibiotics, the demand for natural alternatives to these drugs increases. Natural ingredients, lower risk of complications and even have beneficial side effects. The aim of this study was to determine the antibacterial effect of herbs Reum ribes L and hyssop Hyssopus officinalis is on some pathogenic bacteria.
Methods: After collecting and confirming the scientific name, the methanolic extract of R. ribes L. and H. officinalis plants was prepared and the antimicrobial effects of the extracts by agar well diffusion and disk diffusion , as well as the determination of The minimum bactericidal concentration and the minimum inhibitory concentration (MIC / MBC) were dilution test on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa.
Results: The highest growth inhibitory zone in S. aureus, B. cereus, P. aeruginosa, and E. coli at concentrations of 400 mg / ml, respectively, in the disc method of 13.21, 13.41, 11.2 and10.74 mm and the well method, respectively 13.64, 13.11, 10.67 mm, and 9.38 mm for the R. ribes L extract, and the disc method of 11.74, 10.2, 10.71, and 9.1 mm, and the well method of 12.41, 11.6, 10.2, and 9.9 respectively. 4.3 mm was observed for H. officinalis extract. The results of MBC / MIC showed that the extract of medicinal plants had the highest susceptibility to B. cereus bacteria and the least susceptibility to E. coli.
Conclusion: R. ribes L. and H. officinalis plants have significant inhibitory effects on the growth of pathogenic bacteria in vitro. Therefore, it can be expected that these extracts can be used for the treatment of bacterial infections and are a good alternative to the usual chemical treatments for the treatment of infections.
Samira Mahmoudi, Rozita Nasiri, Abolfazl Jafari Sales,
Volume 7, Issue 4 (12-2019)
Abstract
Background and objectives: Microbial resistance to antibiotics is one of the most common problems in the health care system. Therefore, many efforts have been performed to find new compounds as antimicrobial compounds. This study carried out to investigate the in-vitro antibacterial effect of methanolic extract of peppermint on standard Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa strain.
Material and Methods: In this experimental laboratory study, after collecting and performing pharmacognosy evaluations, methanolic extract of the peppermint plant was prepared and its antimicrobial effects on several bacteria were determined at concentrations of 20 to 400 mg/ml using the agar well diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) methods. The test was repeated five times for each bacterium and the collected data were analyzed using SPSS software.
Results: It was revealed that the methanolic extract of peppermint had antimicrobial effects against standard Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. The highest effect was observed in S. aureus and the least in P. aeruginosa. MIC and MBC of methanol extract on S. aureus, B. cereus, E. coli, and P. aeruginosa were 6.5-15.5, 15.5-25, 50-100, and 100-200 mg/ml, respectively.
Conclusion: The results of this study reveal that peppermint can be considered as an antibacterial medicinal herb and that if the concentration of the extract is higher, its antibacterial properties will also increase. Thus, it can be used as an alternative to the usual chemical drugs in the treatment of infections after evaluating their effects in vitro.
Fatemeh Monirian, Reyhane Abedi, Negar Balmeh, Samira Mahmoudi, Fereshteh Mirzaei Poor,
Volume 8, Issue 3 (10-2020)
Abstract
Background and objective: Some common problems in the health care system are Microbial resistance to antibiotics, the side effects of food additives, and preservatives. Considering the antibiotic resistance of microorganisms and the need to identify new compounds, the present study was conducted to determine the antimicrobial effects of Artemisia extracts.
Material And Method The study was performed in two stages including extraction and determination of antibacterial properties of aqueous, ethanolic, methanolic, acetone/ethanolic, and hydroethanolic extracts of Artemisia on standard Pseudomonas aeruginosa, Streptococcus pyogenes, and oral bacteria sample. The well diffusion method was performed to evaluate the concentration that had an inhibitory and bactericidal effect.
Result: Aqueous extract had an effect on the oral bacteria sample (8 mm), the methanolic extract had an effect on S. pyogenes and oral bacteria sample (15 mm) and finally acetone/ethanolic extracts had antimicrobial properties against S. pyogenes (16 mm). The concentration used for all five extracts was 50 mg/ml and showed an inhibition effect on the growth of S. pyogenes standard strain and oral bacteria sample. The less serial dilutions of extracts were tested but no antibacterial effects were seen. So, 50 mg/ml was the minimum concentration that had an inhibitory and bactericidal effect.
Conclusion: It can be inferred that aqueous, methanolic, and acetone/ethanolic extracts of Artemisia had the highest inhibitory effect on S. pyogenes and the oral bacteria sample. Consequently, by applying different extraction methods and by utilizing different solvents, it may be possible to more efficiently obtain biomaterials with antimicrobial properties from this plant.
Amir Abbas Barzegari, Ahmad Aghaee, Kamran Shahabi,
Volume 11, Issue 2 (10-2023)
Abstract
Background: Promising results were obtained by using medicinal plants for the treatment and prevention of opioid withdrawal syndrome. A native Iranian plant, Echinophora platyloba, has shown analgesic effects that may be mediated through opioid receptors. Thus, the present research evaluated the effects of the ethanolic extract of E. platyloba on the acquisition of morphine dependence in mice.
Methods: Thirty-two male mice were randomly allocated into four groups of eight. Morphine dependence was induced in the mice by subcutaneous administration of morphine (50, 50, and 75 mg/kg)×3 days, plus a single dose of morphine (50 mg/kg) on the fourth day. Withdrawal syndrome was precipitated by intraperitoneal injection of naloxone (4 mg/kg) 2 hours after the last dose of morphine. On days of dependence induction and before each morphine dose, the mice received intraperitoneal injections of saline (10 ml/kg) or plant extract (25, 50, and 75 mg/kg). After the injection of naloxone, the symptoms of withdrawal syndrome in each animal were monitored for 30 minutes.
Results: Administering morphine with this method induced morphine dependence in mice that were treated with saline before morphine. However, the mice that received the plant extract showed a significant decrease in the withdrawal symptoms including the number of jumping (P<0.01), rearing (P<0.05), grooming (P<0.05), and diarrhea (P<0.01) compared with the saline-treated mice.
Conclusion: Administration of the hydro-ethanolic extract of E. platyloba before morphine may inhibit the induction of morphine dependence. Therefore, the plant extract may be considered a therapeutic agent for the prevention of morphine dependence in morphine users.
Vahid Fallahzadeh, Farzaneh Taghian, Khosro Jalali Dehkordi,
Volume 11, Issue 3 (12-2023)
Abstract
Background: Aerobic exercise and ginger after myocardial infarction (MI) modify calcium handling. Ginger has cardioprotective effects on cardiovascular disease. This study assessed the effects of aerobic exercise combined with ginger extract (GE) loaded into chitosan nanoparticles (CNPs) on miRNA-214, Serca2a, and Anp genes and cardiac fibrosis in myocardial infarction (MI) rat models.
Methods: Twenty-five male rats divided into 5 groups were subjected to ginger treatment and exercise. Aerobic exercises (AE) were performed on a rodent treadmill 5 days per week for 6 weeks. The GE-CNPs (500 mg/kg) were orally administered to the rats for 6 weeks. The expressions of miRNA-214, Serca2a, and Anp genes were assessed by real-time polymerase chain reaction (PCR). The histopathological assessments were performed using hematoxylin and eosin (H&E) and Masson's trichrome staining. The serum activities of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) were also measured by ELISA.
Results: The MI model and CNP groups had the highest rate of collagen deposition (P <0.05). The serum activities of both CK-MB and LDH were significantly elevated in the Isop group compared to the control (P<0.05), while following aerobic exercise and ginger treatment, their activity was significantly dropped in the Isop + AE + GE-CNPs group. The expression of miRNA-214 showed a significant increase in GE-CNPs (P <0.01) and GE-CNPs + AE (P <0.001) groups. Serca2a and Anp genes showed significant changes in the GE-CNPs + AE group (P<0.05).
Conclusion: Our findings revealed that aerobic exercise, along with ginger treatment, improved cardiac fibrosis, modulating the expression levels of miRNA-214, Serca2a, and Anp genes and serum levels of MI biomarkers.
