Search published articles


Showing 4 results for Apoptosis
Increased Expression of Bax and Bcl2 Apoptosis Biomarkers in the Heart of Old Female Rats After Interval Training and Curcumin Consumption
Bahareh Yazdanparast Chaharmahali, Mohammad Ali Azarbayjani, Maghsood Peeri, Parvin Farzanegi Arkhazloo,
Volume 6, Issue 4 (12-2018)
Abstract
Background and objectives: Elderly is characterized by decreased cardiac function, which is widely associated with mitochondrial failure in cell death. Elderly causes heart apoptosis. Although the old heart is vulnerable to apoptosis, physical training and some supplements through different mechanisms could be effective in reducing apoptosis. The present study aimed to investigate the effect of interval training on some apoptotic parameters of Bax and Bcl2 cardiomyocytes in old female rats.
 
Methods: In an experimental study, 49 Wistar female rats (2 years old) were randomly assigned into 7 groups: control, saline, curcumin, moderate intensity training, moderate intensity training + curcumin, high intensity training, high intensity training + curcumin. The experimental groups received gavage doses of curcumin 30 mg / kg body weight three days a week via. The saline group also received the same amount of saline. Training groups had moderate and severe exercises on the treadmill for eight weeks, and three sessions per week. Forty-eight hours after the last training session and gavage, the animals were killed and their heart tissues were isolated to determine the levels of Bax and Bcl2.
 
Results: The results indicated that interval training and curcumin consumption could significantly increase the levels of Bcl2 and BAX and the BAX / Bcl2 ratio, the amount of which depends on the intensity of training. Moreover, the combination of training and curcumin had an antagonistic effect.
 
Conclusion: According to the results, physical activity and curcumin separately have a protective effect on the heart tissue; however, these two interventions do not enhance each other’s effect. Hence each one is suggested to be used separately.
 
Study the Effect of Swimming Training and Curcumin on Reduction of BAX and P53 proteins and Increase in BCL2 protein in Heart Tissue of Rats During Withdrawal of Excessive Ethanol Consumption
Mona Abdolhamid Tehrani,
Volume 10, Issue 4 (12-2022)
Abstract
Background and objectives: Epidemiological studies have shown that high dose of ethanol can lead to apoptosis. On the other hand, the consumption of medicinal plants such as turmeric in the diet and performing physical activities are considered as factors to control apoptosis. Therefore, this article aims to mainly evaluate the anti-apoptotic effects of swimming and curcumin interactively during withdrawal of binge ethanol exposure in heart tissues.
Material and Methods: In this study, 40 rats were received ethanol (25% W/V) as gavage for four days every eight hours. Then, 7 days after ethanol withdrawal, they were classified in 5 groups, including: (1) control, (2) curcumin, (3) swimming, (4) swimming with curcumin, and (5) sham. Groups 3 and 4 performed swimming five sessions a week for two weeks, and groups 2 and 4 received 50 mg/kg of curcumin five times a week for two weeks. The amount of BAX, BCL2, and P53 protein and BCL2/BAX ratio were measured using western blot technique. The data were tested using the independent t-test at a significant level (p≥0.05) and two-way ANOVA.
Results: Swimming had a significant effect on reduction of p53 (F=60.051, P<0.0001, η2=0.741), Bax (F=62.594, P<0.0001, η2=0.887) and Bax/Bcl-2 ratio (F=290.591, P<0.0001, η2=0.973), and increase of Bcl-2 (F=150.940, P<0.0001, η2=0.950); Curcumin had a significant effect on decrease of p53 (F=5.513, P=0.029, η2=0.208), Bax (F=66.146, P<0.0001, η2=0.892) and Bax/Bcl-2 ratio (F=260.655, P<0.0001, η2=0.970), and increase of Bcl-2 (F=73.274, P<0.0001, η2=0.902). The results from two-way ANOVA showed the interactive effect of exercise and curcumin on the decrease of BAX (F=35.847, P<0.0001, η2=0.818) and BAX/BCL-2 ratio (F=175.887, P<0.0001, η2=0.956), and the increase of BCL-2 (F=21.205, P=0.002, η2=0.726) were significant; but it had no significant effect on p53 (F=0.000, P=0.999, η2=0.000).
Conclusion: Swimming and curcumin consumption simultaneously can significantly moderate apoptosis caused by ethanol in the heart tissue.

Investigating the Effects of Lactobacillus Plantarum Strain ATCC 8014 on Gene Expression of NF-ĸB, TLR-4, and BCL-2 in Oral Rat Cancer Induced by 4-Nitroquioline 1-Oxide
Vahideh Faghani Zadeh, Nazila Arbab Soleimani, Ayyoob Khosravi, Mohammad Mahdi Forghanifard,
Volume 10, Issue 4 (12-2022)
Abstract
Background and Objectives: Oral cancer relates to oral and intestinal microbiota composition. Lactobacillus species are considered probiotic bacteria due to their ability to modulate human immune responses and therapeutic effects. This study aimed to investigate the effects of Lactobacillus plantarum (L. plantarum) on TLR4 gene expression and its downstream pathways in a mouse model of oral cancer.
Material and Methods: This experimental study was conducted in the Stem Cell Research Center, Faculty of Modern Technologies, Golestan University of Medical Sciences, Gorgan, Iran, in 2021. L. plantarum strain ATCC 8014 was used in this study. Twenty-eight male Wistar rats were divided into four groups (n = 7). 4-Nitroquinoline 1-oxide (4NQO) was used to establish oral cancer in rats. A pathological examination was adopted to confirm cancer establishment. Rats were treated with probiotic L. plantarum before and after cancer development. After extracting RNA from blood and synthesizing cDNA, the ability of lactobacilli to modulate the expression of TLR4 genes was investigated by Real-Time PCR Methods (RT-PCR).
Results: In the present study, it was observed that after causing cancer and treating the animal with L. plantarum, the expression of the TLR4 gene decreased significantly (P-value < 0.05), which might, in turn, affect the downstream pathways, which included the decrease in the expression of BCL-2 and NF-ĸB genes. Accordingly, the expression of the NF-ĸB gene was significantly reduced in rats received L. plantarumgavaged for two weeks after cancer induction.
Conclusion: According to the obtained results, probiotic L. plantarum significantly affects the gene expression of NF-ĸB and TLR4 in cancerous rats. It was also shown that L. plantarum was more efficient in reducing TLR-4 and NF-ĸB genes expression when it was gavaged for 14 days after tumor induction.

The effect of eight weeks of high-intensity interval training with spirulina supplementation on apoptotic markers in the hippocampal tissue of high-fat diet-induced obese rats
Soraya Pourasghari Siah Astalkhi , Seyed Morteza Tayebi, Lida Moradi, Rasoul Eslami,
Volume 12, Issue 3 (12-2024)
Abstract
Background: Obesity leads to neuronal apoptosis and cognitive impairment by increasing inflammation and oxidative stress. Although the beneficial role of exercise and antioxidant supplements have been reported separately, their interactive effect on neuronal apoptosis in obesity is still not well known. Therefore, this study aimed to investigate the effect of eight weeks of HIIT with Sp supplementation on apoptotic markers in the hippocampal tissue of high-fat diet-(HFD) induced obese rats.
Methods: In this experimental study, 24 obese male Sprague-Dawley rats (60% fat, 20% protein, and 20% carbohydrates) were randomly divided into four groups of six rats, including 1) HFD, 2) HFD+Sp, 3) HFD+HIIT, and 4) HFD+HIIT+Sp. Also, six healthy rats were selected as the HC group to investigate the effects of HFD on research variables. Subsequently, HIIT was performed for eight weeks, three sessions per week with an intensity of 80-110% of maximum running speed and 2-8 high-intensity intervals. Also, Sp supplement was taken orally during this period with a dose of 100 mg/kg/day. Bax and BCL-2 levels were measured with qRT-PCR method. One-way analysis of variance and Tukey's post-hoc tests were used for statistical analysis of data (p ≤0.05).
Results: In the HFD+HIIT and HFD+HIIT+Sp groups, Bax levels and Bax/BCL-2 ratio were lower and BCL-2 levels were higher than the HFD group (p=0.001). In the HFD+Sp group, BCL-2 levels were higher and Bax/BCL-2 ratios were lower than HFD group (p=0.008). Also, in the HFD+HIIT and HFD+HIIT+Sp groups, Bax levels and Bax/BCL ratio were lower and BCL-2 levels were higher than HFD+Sp group (p≤0.05). In addition, in the HFD+HIIT+Sp group, Bax levels were lower and BCL-2 levels were higher than in the HFD+HIIT group (p≤0.05).
Conclusion: It seems that although HIIT and Sp supplement separately have an anti-apoptotic effect, their interactive effect on inhibiting neuronal apoptosis in brain tissue in obesity conditions is more obvious than the effect of each one alone.
 

Page 1 from 1     

© 2025 CC BY-NC 4.0 | Jorjani Biomedicine Journal

Designed & Developed by : Yektaweb