Jorjani Biomedicine Journal

Background and Objective: Alzheimer's disease is a common cause of dementia and reduces progressive perception, memory, and tasks.  This study aimed was to investigate the effects of interval training and octopamine extract on NLRP-1 and NLRP-3 in brain tissue of rats with Alzheimer's disease.
Material and Methods: The research method was experimental with a post-test design. A total of 42 male Wistar rats, all of which were eight weeks old, were obtained. Rats were randomly divided into 5 groups (healthy control, Alzheimer's sham, Alzheimer's+ interval training, Alzheimer's+ octopamine supplementation, Alzheimer's+ interval training+ octopamine supplementation). Alzheimer's induction was performed with beta-amyloid peptide 1-14. The training protocol was intense periodic. Octopamine was supplemented by intra-peritoneal injection. Morris's blue maze test was used for the spatial memory test. Seventy-two hours after the last training session, anesthetized rats and hippocampus were quickly extracted. A one-way ANOVA test was used to estimate intergroup differences after Alzheimer's induction.
Results: The results of one-way ANOVA showed a significant difference between the groups in NLRP-1 and NLRP-3 mRNA. Bonferroni test confirmed significant between the control group and Alzheimer's groups (P≤0.05).
Conclusion: The changes were considered to be synergistic of the beneficial effects of physical activity and octopamine in preventing or reducing the harmful effects of pathological conditions. Exercise and supplementation seem to be effective in the relationship between inflammatory and neurotrophic factors in neurological disorders.

Background: Recently, the role of sports training and medicinal plants in the expression of genes has been considered to prevent the progression of diseases. The purpose of this research was to investigate the role of weight training and oleander extract on interferon regulatory factor 8 (IRF8) and cathepsin S (CTSS) gene expression in the soleus muscle of Alzheimer's disease (AD) model mice.
Methods: Fifty-five male Wistar rats were randomly divided into 5 groups: healthy control group, AD control group, AD resistance training group, AD group with the supplement of Melilotus officinalis, and AD resistance training group + Melilotus officinalis supplement. Trimethyltin-induced AD was induced. In the resistance training protocol, a weight was attached to the tail of the rats, and they had to lift this weight on a ladder with 26 steps. Melilotus officinalis was injected intraperitoneally as a supplement for 6 weeks with a dose of 300 mmol/kg. Seventy-two hours after the last training session, the rats were anesthetized, and the hippocampal tissue was immediately extracted, frozen, and analyzed. A two-way analysis of variance was used to estimate the differences between groups in control and experimental AD mice.
Results: There was a significant increase in the expression level of interferon-regulating factor 8 and cathepsin S genes in the AD group compared to the control group. The results of Bonferroni's post-hoc test showed that in the AD group + resistance training + Melilotus officinalis, a significant decrease was observed compared to the AD group (P≤0.05).
Conclusion: Resistance training and the Melilotus officinalis extract with antioxidant mechanisms can affect CTSS and IRF8 gene expression.